Morroniside Promotes PGC-1α-Mediated Cholesterol Efflux in Sodium Palmitate or High Glucose-Induced Mouse Renal Tubular Epithelial Cells

Figure 7

Silencing PGC-1α significantly suppressed the inhibitory effect of MOR on PA-induced total cholesterol levels in mRTECs. (a) Western blot analysis of PGC-1α, LXR, ABCA1, ABCG1, and ApoE expressions in mRTECs cultured for 36 hours and then exposed to PA (sodium palmitate 50 μM), PA+M (), PA+M+P (), or PA+M+C (). (b) Real-time PCR analysis of PGC-1α and LXR expressions in mRTECs cultured for 24 hours. Real-time PCR analysis of ABCA1, ABCG1, and ApoE expressions in mRTECs cultured for 36 hours. (c) Colorimetric analysis of TC and TG levels in mRTECs cultured with PGC1α-siRNA for 36 hours. Data represent the (). , , and compared with the PA group; ^#, ^##, and ^### compared with the PA+M+P group.

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