The Potential Genes Mediate the Pathogenicity of Allogeneic CD4⁺T Cell in aGVHD Mouse Model

Figure 6

The verification of the expression of hub genes in vivo. (a) The hub genes from allospleen and autospleen were verified in GSE126518. (b) The hub genes were verified from different tissues by qRT-PCR. CXCL7 deposition was detected in target areas of the spleen in aGVHD mouse animals. (c) Quantification of the histology staining shown. At the left is one representative section per group. At the right is the number of CXCL7 per scale in the spleen. CXCL7 were detected by colocalization of CXCL7 (green) and DAPI (blue). CXCL7 deposition was quantified on a 0-3 scale to determine the amount of antibodies in the tissues. (d) Representative spleen histology staining revealing the expression of CXCL7 (brown) 7 d after HSCT in auto-HSCT and allo-HSCT mice. Cells were stained with CXCL7 (brown). Bars: 50 or 200 μm. and . The -axis is the log2 scale of the expression level of hub genes. Each experiment was performed at least three times, and the results were presented as .

(a)

(b)

(c)

(d)