hsa_circ_0023305 Enhances Laryngeal Squamous Cell Carcinoma Progression and Modulates TRPM7 via miR-218-5p Sponging

Figure 2

Downregulation of hsa_circ_0023305 suppresses LSCC cell proliferation and tumor growth. (a) hsa_circ_0023305 expression in Hep-2 and AMC-HN-8 cells transfected with sh-hsa_circ_0023305 or sh-NC. Data are presented as the ; . (b, c) CCK8 analysis shows the proliferative capacity of both Hep-2 and AMC-HN-8 cells after transfection with sh-hsa_circ_0023305 or sh-NC. Data are presented as the ; vs. sh-NC. (d, e) Plate cloning experiments were performed to detect the number of LSCC cells transfected with sh-hsa_circ_0023305 or sh-NC in both Hep-2 and AMC-HN-8 cells. Data are presented as the ; . (f) AMC-HN-8 cells were transfected with sh-hsa_circ_0023305 or sh-NC and subcutaneously injected into nude mice. (g, h) Tumor growth curves and tumor weight were analyzed. Data are presented as the ; vs. sh-NC. (i, j) Immunohistochemistry for the percentage of Ki67-positive cells. The relative Ki67 expression was calculated. Data are presented as the ; vs. sh-NC.

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