hsa_circ_0023305 Enhances Laryngeal Squamous Cell Carcinoma Progression and Modulates TRPM7 via miR-218-5p Sponging

Figure 4

The miR-218-5p and TRPM7 are downstream targets of hsa_circ_0023305. (a) The luciferase activity of hsa_circ_0023305 in HEK293T cells transfected with different miRNA mimics, which are putative binding sites for the hsa_circ_0023305 sequence. Luciferase activity was normalized by Renilla luciferase activity. (b) Prediction of binding sites of miR-218-5p in hsa_circ_0023305. The MUT version of hsa_circ_0023305 is presented. (c) Relative luciferase activity was determined 48 h after transfection of HEK293T cells with miR-218-5p mimic/NC or hsa_circ_0023305 WT/Mut. Data are presented as the . . (d) Prediction of binding sites of miR-218-5p within the 3-UTR of TRPM7. The MUT version of 3-UTR-TRPM7 is shown. (e) Relative luciferase activity was determined 48 h after transfection of HEK293T cells with miR-218-5p mimic/NC or 3-UTR-TRPM7 WT/Mut. Data are presented as the . .

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