Research Article
Diagnostic Accuracy of Metagenomic Next-Generation Sequencing in Sputum-Scarce or Smear-Negative Cases with Suspected Pulmonary Tuberculosis
Table 2
Diagnostic performance of mNGS, conventional tests, and the T-SPOT.TB assay in PTB.
| | PTB | Non-PTB | Sensitivity (95% CI) | Specificity (95% CI) | PPV (95% CI) | NPV (95% CI) |
| | mNGS | | | 89.13 (75.64, 95.93) | 98.36 (90.02, 99.91) | 97.62 (85.91, 99.88) | 92.31 (82.25, 97.13) | | Positive | 41 | 1 | | | | | | Negative | 5 | 60 | | | | | | Conventional tests | | | 67.39 (51.86, 80.03) | 95.08 (85.40, 98.72) | 91.18 (75.19, 97.69) | 79.45 (68.07, 87.68) | | Positive | 31 | 3 | | | | | | Negative | 15 | 58 | | | | | | T-SPOT.TB assay | | | 76.09 (60.90, 86.92) | 65.57 (52.22, 76.96) | 62.50 (48.52, 74.77) | 78.43 (64.30, 88.25) | | Positive | 35 | 21 | | | | | | Negative | 11 | 40 | | | | | | Conventional tests combined with T-SPOT.TB assay | | | 91.30 (78.31, 97.18) | 63.93 (50.57, 75.54) | 65.63 (52.61, 76.75) | 90.70 (76.95, 96.98) | | Positive | 42 | 22 | | | | | | Negative | 4 | 39 | | | | |
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Abbreviations: mNGS: metagenomic next-generation sequencing; PTB: pulmonary tuberculosis; Non-PTB: non-pulmonary tuberculosis; PPV: positive predictive value; NPV: negative predictive value.
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