Diagnostic Accuracy of Metagenomic Next-Generation Sequencing in Sputum-Scarce or Smear-Negative Cases with Suspected Pulmonary Tuberculosis

Table 3

Diagnostic performance of mNGS in BALF and lung tissue biopsy specimens.

mNGS	PTB	Non-PTB	Sensitivity (95% CI)	Specificity (95% CI)	PPV (95% CI)	NPV (95% CI)
BALF			90.63 (73.83, 97.55)	97.83 (87.03, 99.89)	96.67 (80.95, 99.83)	93.75 (81.80, 98.37)
Positive	29	1
Negative	3	45
Lung biopsy specimens			85.71 (56.15, 97.49)	93.33 (66.03,99.65)	92.31 (62.09, 99.60)	87.5 (60.41, 97.80)
Positive	12	1
Negative	2	14

Abbreviations: mNGS: metagenomic next-generation sequencing; BALF: bronchoalveolar lavage fluid; PTB: pulmonary tuberculosis; Non-PTB: non-pulmonary tuberculosis; PPV: positive predictive value; NPV: negative predictive value.