[Retracted] Isovitexin Suppresses Stemness of Lung Cancer Stem-Like Cells through Blockage of MnSOD/CaMKII/AMPK Signaling and Glycolysis Inhibition

Figure 1

Identification of LCSLCs from human non-small-cell lung cancer H460 cell line.(a) The sphere-forming rate of generations 1-4 of H460 sphere-forming cells consecutively subcultured in stem cell-conditioned medium. (b) The colony-forming rate of parental H460 cells and its generation 2 sphere-forming cells, which were presumed lung cancer stem-like cells (LCSCs), in soft agar medium. The migratory (c) and invasive (d) capability of parental H460 cells and presumed LCSLCs. The protein expression of CD133, CD44, and ALDH1 (e); Nanog and Bmi1 (f); and MnSOD, p-AMPK, AMPK, p-CaMkII, and CaMkII (g) in parental H460 cells and presumed LCSLCs. (h) The glycolysis in H460 cells and LCSLCs was assessed by determination of the L-lactate metabolism rate. vs. control ().

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)