[Retracted] Isovitexin Suppresses Stemness of Lung Cancer Stem-Like Cells through Blockage of MnSOD/CaMKII/AMPK Signaling and Glycolysis Inhibition

Figure 5

The combination of isovitexin and MnSOD shRNA suppressed stemness and glycolysis of LCSLCs. The LCSLCs were firstly infected with recombinant adenovirus packaging MnSOD shRNA (Ad-sh MnSOD) or GFP (Ad-GFP) and then treated with isovitexin (0, 10 μg/mL) as indicated. The combination of isovitexin and MnSOD shRNA led to the maximal inhibition of sphere-forming (a), colony-forming (b), migratory (c), and invasive (d) ability of LCSLCs. The expression of CD133, CD44, and ALDH1 (e); Nanog and Bmi1 (f); and MnSOD, p-AMPK, and p-CaMkII (g) decreased the most in LCSLCs treated with isovitexin and MnSOD shRNA. (h) Isovitexin and MnSOD shRNA synergistically suppressed the glycolysis in LCSLCs. vs. Ad-GFP; ^# vs. 10 μg/mL isovitexin treatment ().

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