[Retracted] FXYD6 Regulates Chemosensitivity by Mediating the Expression of Na+/K+-ATPase α1 and Affecting Cell Autophagy and Apoptosis in Colorectal Cancer

Figure 2

FXYD6 regulated chemoresistance through mediating CRC cell apoptosis. (a) FXYD6 silenced cells and FXYD6 overexpressed cells were successfully constructed in SW620 and SW620/Iri cells. The expression level of FXYD6 was measured by RT-PCR. (b) MTT assay was carried out to confirm drug concentration of Iri and Oxa when the cell survival rate changed significantly compared to the nontreated group. (c) Flow cytometric analysis was performed in FXYD6 silenced SW620 cells and FXYD6 overexpressed SW620/Iri cells. The apoptosis rate was analyzed by GraphPad Prism 7. LR: early apoptosis; UR: late apoptosis. (d) Western blotting was performed on apoptosis element expression in FXYD6 silenced SW620 cells and FXYD6 overexpressed SW620/Iri cells. Relative abundance was analyzed by the ImageJ program.

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