[Retracted] FXYD6 Regulates Chemosensitivity by Mediating the Expression of Na+/K+-ATPase α1 and Affecting Cell Autophagy and Apoptosis in Colorectal Cancer

Figure 3

FXYD6 regulated chemoresistance through mediating CRC cell autophagy. (a) SW620 and SW620/Iri cells stably expressed RFP (red puncta) after cotransfection. Autophagic puncta were documented by a fluorescence microscope (200x magnification). Scale bar: 20 μm. Autophagic puncta were counted in 20 cells which were used to quantify. (b) Western blotting was conducted on autophagy elements in FXYD6 silenced and overexpressed cells, respectively. Relative abundance was analyzed by the ImageJ program; LC3I was the internal control of LC3II; β-actin was the internal control of Beclin1 and P62. (c) MTT assays were conducted to measure the cell survival rate when autophagy was activated or inhibited. Rapa is an autophagic stimulator, and 3-MA is an autophagic inhibitor; PBS and DMSO were used as the control group, respectively. (d) Flow cytometric analysis was used to test apoptosis when autophagy was activated with Rapa or inhibited with 3-MA. The apoptosis rate was analyzed by GraphPad Prism 7. LR: early apoptosis; UR: late apoptosis.

(a)

(b)

(c)

(d)