[Retracted] Therapeutical Significance of Serpina3n Subsequent Cerebral Ischemia via Cytotoxic Granzyme B Inactivation

Figure 6

(a) Immunoblot of brain lysates probed with granzyme B marked bands at ~32 kDa and ~80 kDa representing endogenous granzyme B and Serpina3n-granzyme B complex, respectively. (b) Immunoblot probed with c-Myc exhibited bands at ~48 kDa and ~80 kDa representing recombinant Serpina3n and Serpina3n-granzyme B complex, respectively. (c) Delivery of exogenous proteins to the focal region was confirmed by extensive overlaid fluorescence of Serpina3n and reporter c-Myc among treatment group, while no such overlaid staining appeared in ischemic-untreated group. (d) Fluorometric assay of granzyme B with brain lysates derived from different treatment groups demonstrating significant inhibitory effect of Serpina3n in vivo. The data is expressed as and analyzed for statistical significance using two-way ANOVA with Bonferroni post hoc test taking as significant value (, , , and vs. ischemic untreated).

(a)

(b)

(c)

(d)