M2 Macrophage-Derived Exosomes Inhibit Apoptosis of HUVEC Cell through Regulating miR-221-3p Expression

Figure 9

Effect of miR-221-3p in the HUVEC inflammatory injury model. (a, c) Cell apoptosis assay was determined using Annexin V/PI staining followed by flow cytometry (). (b) Cell proliferation was determined by CCK8 assay (). (d) qRT-PCR analyses of inflammatory factors IL-6, IL-10, IL-1β, and TNF-α mRNA in HUVECs (). (e) Representative western blots of apoptosis-related proteins (Caspase-3, Bcl2, and c-myc) and Grb10 protein expression in HUVEC cells after liposome transfection (). Compared with control group. , , .

(a)

(b)

(c)

(d)

(e)