Repression of the Antioxidant Pyrroloquinoline Quinone in Skin Aging Induced by Bmi-1 Deficiency

<div>The effect of PQQ on redox balance in the skin of Bmi-1<sup>-/-</sup> mice. (a) Cellular ROS levels in the skin from 8-week-old WT, Bmi-1<sup>-/-</sup>, and Bmi-1<sup>-/-</sup>+PQQ mice were analyzed by flow cytometry. (b) ROS levels were calculated. Biochemistry analysis of skin extracts from 8-week-old WT, Bmi-1<sup>-/-</sup>, and Bmi-1<sup>-/-</sup>+PQQ mice for (c) H<sub>2</sub>O<sub>2</sub> levels or (d) TAOC. (e) Western blot analysis of protein level for SOD2 in the skin from the WT, Bmi-1<sup>-/-</sup>, and Bmi-1<sup>-/-</sup>+PQQ mice. <i>β</i>-Actin was used as a loading control. (f) SOD2 protein levels relative to <i>β</i>-actin protein levels were determined using densitometric analysis and expressed relative to WT. 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34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg> versus Bmi-1<sup>-/-</sup> mice.</div>

BioMed Research International

fig4

Figure 4

Figure 4: Repression of the Antioxidant Pyrroloquinoline Quinone in Skin Aging Induced by Bmi-1 Deficiency 