SH005S7 Overcomes Primary and Acquired Resistance of Non-Small Cell Lung Cancer by Combined MET/EGFR/HER3 Inhibition

Figure 5

SH005S7 blocks the growth of xenographic implanted lung cancer in nude mice. HCC827GR cells () were transplanted subcutaneously to BALB/c (nu/nu) mice. Animals were randomly assigned to each group; saline (vehicle) or SH005S7 was administered orally for 39 days. Mice were sacrificed 1 day after last administration. (a) Growth curves of tumor volume in xenograft mice. (b) Tumor weight removed from the mice. (c) Body weight during the SH005S7 oral administration period. (d, e) Representative images in tumor sections and quantitative analysis of cell proliferation marker PCNA and angiogenesis marker CD31. IHC magnification ×400. (f) Western blot analysis of proliferation marker (Ki-67 and PCNA) in HCC827GR-derived xenografts treated with vehicle or SH005S7 three times a week. (g) Macroscopic images of vascular irrigation in tumors derived from HCC827GR cells and quantitative presentation of capillary density.

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