Application of Porcine Kidney-Derived Extracellular Matrix as Coating, Hydrogel, and Scaffold Material for Renal Proximal Tubular Epithelial Cell

Figure 1

Effects of renal ECM as a coating material for human renal proximal tubular epithelial (RPTE) cell morphology, proliferation rate, gene expression, and senescence. Cells were cultured on the renal ECM-coated plates for 7 days. Coating materials, such as Matrigel, rat tail collagen type 1, gelatin, fibrinogen, and thrombin were compared. (a) Cell morphology on uncoated plate; (b) cell morphology on coated plate and cell assembly (arrowhead); (c) cell proliferation rate with MTT assay; (d–f) gene expression for human kidney-specific progenitors (CD133, CD24), representative renal progenitor (Pax2), general physiological features (pan-Cytokeratin), endocytosis receptor (Megalin), ion channels (Muc-1), water channel (AQP1), sodium-dependent glucose transport system (SGLT2), dedifferentiation from final differentiated epithelium to renal progenitor, and epithelial-to-mesenchymal transition (EMT) with E-cadherin, β-catenin, and Vimentin; (g) cell senescence assay. Renal ECM: porcine kidney-derived extracellular matrix; original magnification, 200; scale bar, 30 μm. ←, swollen cell body; ▲, dendrite production; , bipolar cell shape. Different letters above bars indicate significant differences ().

(a)

(b)

(c)

(d)

(e)

(f)

(g)