Application of Porcine Kidney-Derived Extracellular Matrix as Coating, Hydrogel, and Scaffold Material for Renal Proximal Tubular Epithelial Cell

Figure 2

Effects of renal ECM concentration on human RPTE cell morphology, proliferation rate, and gene expression. Cells were cultured on the renal ECM-coated plate with various concentrations for 7 days. (a) Cell morphology, (b) cell proliferation rate, and (c–e) gene expression for CD133, CD24, Pax2, pan-Cytokeratin, Megalin, Muc-1, AQP1, SGLT2, E-cadherin, β-catenin, and Vimentin. ECM: porcine kidney-derived extracellular matrix; renal ECM concentration 10, 30, 50, or 100 μg/μL of each well of a 48-well plate. Original magnification, 200x; scale bar, 30 μm; ←, swollen cell body; ▲, dendrite production; §, swirled pattern; , mass formation. Different letters above bars indicate significant differences ().

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