Application of Porcine Kidney-Derived Extracellular Matrix as Coating, Hydrogel, and Scaffold Material for Renal Proximal Tubular Epithelial Cell

Figure 4

Effects of renal ECM as a scaffold material for human RPTE cell morphology, chemical composition, microstructure, proliferation rate, and gene expression. Cells were cultured on the scaffold (renal ECM concentration, 1 mg/scaffold) for 7 days. The collagen scaffold made from rat tail collagen type I scaffold was used as a control. (a) Scaffold morphology, (b) chemical composition analysis with FTIR spectroscopy, (c) SEM images, (d) cell proliferation rate, (e) cell distribution analysis with DAPI, and (f) gene expression for CD133, CD24, Pax2, pan-Cytokeratin, Megalin, Muc-1, AQP1, SGLT2, E-cadherin, β-catenin, and Vimentin. ECM: porcine kidney-derived extracellular matrix; collagen 1: scaffold made with rat tail collagen type I; renal ECM: scaffold made with renal ECM. Original magnification, 1000x; scale bar, 50 μm. Different letters above bars indicate significant differences ().

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