Application of Porcine Kidney-Derived Extracellular Matrix as Coating, Hydrogel, and Scaffold Material for Renal Proximal Tubular Epithelial Cell

Table 1

The primer sequences.

Gene	Function	Sequences
CD24	Human kidney-specific progenitors	5-tca aca gcc agt ctc tc gt-3; 5-gac gtt tct tgg cct gag tc-3
CD133		5-ttc ttg acc gac tga gac cc-3; 5-tgg tct cct tga tcg ctg tt-3
Paired box gene 2 (Pax2)	Representative renal progenitor	5-tct ctc ctc tcc gct tct ct-3; 5-cga cag aga cgg aga ac-3
Pan-Cytokeratin	General physiological feature	5-act tga caa ctt gca gca gg-3; 5-caa tga tgc tgt cca ggt cg-3
Megalin	Endocytosis receptor	5-ctt gca act atc cga cct gc-3; 5-gga ccg ctt tca cat cca tc-3
Mucin-1 (Muc-1)	Ion channels	5-tcc ttt ctc tgc cca gtc tg-3; 5-caa cca gaa cac aga cca gc-3
Aquaporin 1 (AQP1)	Water channel	5-cca tca caa ctc tcc cca ct-3; 5-cca tca caa ctc tcc cca ct-3
Sodium glucose co-transporter 2 (SGLT2)	Sodium-dependent glucose transport system	5-ctc tct tcg cca gca aca tc-3; 5-cca ctc gaa tcc agc aac ag-3
Epithelial cadherin (e-cadherin)	Epithelial-to-mesenchymal transition markers	5-aag ggg tct gtc atg gaa gg-3; 5-ggtgtt cac atc atc gtc cg-3
β-Catenin		5-gag ggt acg agc tgc tat gt-3; 5-aac gct gga cat tag tgg ga-3
Vimentin		5-ctt tgc cgt tga agc tgc ta-3; 5-acg agc cat ttc ctc ctt ca-3