Research Article
SARS-CoV-2 Induces Expression of Cytokine and MUC5AC/5B in Human Nasal Epithelial Cell through ACE 2 Receptor
Table 1
Primer used for polymerase chain reaction.
| | Gene | Primary sequence | Annealing temperature (°C) | Product size (bp) |
| | IL-1β | F: 5-ATG CAC CTG TAC GAT CAC TG-3
R: 5-ACA AAG GAC ATG GAG AAC ACC-3 | 60 | 142 | | IL-6 | F: 5-GGT ACA TCC TCG ACG GCA TCT-3
R: 5-GTG CCT CTT TGC TGC TTT CAC-3 | 60 | 145 | | IL-8 | F: 5-ATG ACT TCC AAG CTG GCC GTG GCT-3
R: 5-TCT CAG CCC TCT TCA AAA ACT TCT C-3 | 60 | 284 | | MUC5AC | F: 5-TCA ACG GAG ACT GCG AGT ACA C-3
R: 5-CTT GAT GGC CTT GGA GCA-3 | 60 | 130 | | MUC5B | F: 5-CAC ATC CAC CCT TCC AAC-3
R: 5-GGC TCA TTG TCG TCT CTG-3 | 60 | 245 | | GAPDH | F: 5-CCT CCA AGG AGT AAG ACC CC-3
R: 5-AGG GGT CTA CAT GGC AAC TG-3 | 60 | 145 |
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