BioMed Research International

Research Article

[Retracted] Antihypertensive Activity in High Salt-Induced Hypertensive Rats and LC-MS/MS-Based Phytochemical Profiling of Melia azedarach L. (Meliaceae) Leaves

Table 1

Phytochemical profile of M. azedarach leaf extract and fractions using tandem mass analysis.


Compound No.	Rt	[M-H]^-m/z	MS2 fragmentation ion [M-H]^-	Dereplication results	Exact Mass (g/mol)	Molecular Formula

Crude extract of M. azedarach leaves

1	4.80	395.11	377.72, 374.95, 348.68 (100), 312.58	3,4’,5,6,7-pentamethoxyflavone	372.12	C₂₀H₂₀O₇
2	5.22	341.10	322.96 (100), 320.64, 300.58	(8,8 dimethyl-2,10-dioxo-9H- pyrano [2,3-f] chromen-9-yl)(Z)-2-methylbut-2-enoate	342.11	C₁₉H₁₈O₆
3	5.96	449.22	426.83, 377.78, 232.47, 222.48, 151.831 (100)	Strophanthidine	404.21	C₂₃H₃₂O₆
4	4.06	464.38	442.84, 432.77, 414.85, 342.58, 298.31(100)	Isoquercetin	464.09	C₂₁H₂₀O₁₂
5	4.06	623.16	623.80, 605.66, 590.46, 579.43, 563.40 (100), 536.39, 516.33, 492.32, 477.26, 314.61, 299.51	Isorhamnetin 3-O-rutinoside	624.16	C₂₈H₃₂O₁₆
6	4.04	593.15	413.07, 314.64, 284.52, 276.68 (100), 240.36	Kaempferol 3-O-[2″-O (glycopyranoside)]-rhamnopyranoside	594.15	C₂₇H₃₀O₁₅
7	4.1	593.20	475.21, 356.76, 326.65, 284.53 (100), 228.38	Kaempferol 3-O-rutinoside	594.15	C₂₇H₃₀O₁₅
8	4.06	593.15	413.07, 314.64, 284.52, 276.68 (100), 240.36	Kaempferol 7-O-neohesperidoside	594.15	C₂₇H₃₀O₁₅
9	4.10	594.16	284.46 (100), 226.53	Keracyanin	630.13	C₂₇H₃₁ClO₁₅
10	6.24	755.44	593.47, 575.42 (100), 477.07, 431.07, 413.04, 276.58	Quercetin 3-O-[2″-O-(6″-O-p-coumaroyl)-β-D-glucopyranosyl]-α-L-rhamnopyranoside	756.19	C₃₆H₃₆O₁₈
11	8.03	987.52	948.95, 729.61, 654.46, 431.01, 414.80 396.82 (100)	Soyasaponin B	942.51	C₄₈H₇₈O₁₈
12	16.60	675.41	415.100 , 397.105 (100)	DGMG 18:3	676.36	C₃₃H₅₆O₁₄
13	3.88	609.15	573.43, 561.42, 518.31, 501.27, 429.07, 300.54 (100), 292.68	Rutin	610.15	C₂₇H₃₀O₁₆
14	4.66	712.40	550.63, 523.60 (100)	Soyacerebroside 1	713.54	C₄₀H₇₅NO₉
15	3.75	755.21	709.90, 609.38, 593.46, 575.42 (100), 477.05, 431.07, 413.04, 276.58	Quercetin 3-O-[2″-O-(6'''-O-p-coumaroyl)-β-D-glucopyranosyl]-α-L-rhamnopyranoside	756.19	C₃₆H₃₆O₁₈

n-Hexane fraction of M. azedarach leaves

4	4.06	463.09	442.84, 432.77, 414.85, 342.58, 298.31(100)	Isoquercetin	464.09	C₂₁H₂₀O₁₂
8	4.04	593.15	413.07, 314.64, 284.52, 276.68 (100), 240.36	Kaempferol 7-O-neohesperidoside	594.15	C₂₇H₃₀O₁₅
14	4.66	712.40	550.63, 523.60 (100)	Soyacerebroside I	713.54	C₄₀H₇₅NO₉
16	3.88	609.15	573.43, 561.42, 518.31, 501.27, 429.09, 300.54 (100), 292.68	Quercetin 3-O-neohesperidoside	610.15	C₂₇H₃₀O₁₆
17	6.62	307.19	278.54, 262.57, 260.39, 198.318 (100), 124.00	Fatty acid 18:4	308.19	C₁₈H₂₈O₄
18	4.21	559.31	397.044, 380.172, 378. 95, 350.967, 160.076, 158.104 (100)	MGMG 18:3	560.0	C₂₇H₄₆O₉
19	6.97	721.36	466.39, 401.19, 326.81, 254.53 (100), 240.64	DGMG 18:3	676.797	C₃₃H₅₆O₁₄
20	16.60	675.36	415.100, 397.105 (100)	DGMG 18:3	676.36	C₃₃H₅₆O₁₄

Chloroform fraction of M. azedarach leaves

8	4.04	593.15	413.07, 314.64, 284.52, 276.68 (100), 240.36	Kaempferol-7-neohesperidoside	594.15	C₂₇H₃₀O₁₅
21	1.15	301.04	254.27 (100), 137.923, 136.81, 122.922	Quercetin	302.04	C₁₅H₁₀O₇
22	6.13	697.32	515.079, 415.042 (100), 326.824, 278.54, 212.211	Momordicoside	696.40	C₃₇H₆₀O₁₂
23	3.75	755.21	709.90, 609.38, 593.46, 575.42 (100), 477.05, 413.04, 431.07, 276.58	Quercetin 3-O-[2-O-6-z-p-coumaroyl-glucopyranoside]-rhamnopyranoside	756.19	C₃₆H₃₆O₁₈
14	4.66	712.40	550.63, 523.60 (100)	Soyacerebroside I	713.54	C₄₀H₇₅NO₉
9	4.10	594.16	284.46 (100), 226.53	Keracyanin	630.13	C₂₇H₃₁ClO₁₅
24	4.61	593.19	549.42, 431.22, 366.813, 284.53 (100), 206.206, 168.208	Isosakuranetin-7-O-neohesperidoside	594.19	C₂₈H₃₄O₁₄

Ethyl acetate fraction of M. azedarach leaves

4	4.06	463.38	442.84, 432.77, 414.85, 342.58, 298.31(100)	Isoquercetin	464.09	C₂₁H₂₀O₁₂
25	4.04	593.15	413.07, 314.64, 284.52, 276.68 (100), 240.36	Kaempferol 3-O-[2-O-(glucopyranoside)] rhamnopyranoside	594.15	C₂₇H₃₀O₁₅

n-Butanol fraction of M. azedarach leaves

26	3.84	739.21	559.43, 558.47, 430.97, 326.66, 284.47 (100)	Robinin	740.21	C₃₃H₄₀O₁₉
27	3.81	625.14	462.08, 444.11, 358.90, 315.53 (100), 270.31	Isorhamnetin-3-O-galactoside-6''-rhamnoside	624.16	C₂₈H₃₂O₁₆
28	4.1	593.20	475.21, 356.76, 326.65, 285.44, 276.68 (100), 228.38	Nicotiflorin	594.15	C₂₇H₃₀O₁₅
15	3.73	755.18	609.387 (100), 489.173, 342.72, 300.56, 298.603, 270.505	Quercetin 3-O-[2''-O-(6'''-O-p-coumaroyl)-β-D-glucopyranosyl]-α-L-rhamnopyranoside	756.19	C₃₆H₃₆O₁₈
13	3.88	609.15	573.43, 561.42, 518.31, 501.27, 429.07, 300.54 (100), 292.68	Rutoside (rutin)	610.15	C₂₇H₃₀O₁₆
29	10.49	574.45	427.168, 349.22, 332.84 (100), 265.761, 246.24, 240.59	Sulfobacin B	575.45	C₃₂H₆₅NO₅S

Aqueous fraction of M. azedarach leaves

23	3.71	755.18	609.387 (100), 489.173, 342.72, 300.56, 298.603, 270.505	Quercetin 3-O-[2-O-6-z-p-coumaroyl-glucopyranoside]-rhamnopyranoside	756.7	C₃₆H₃₆O₁₈
13	3.88	609.15	573.43, 561.42, 518.31, 501.27, 429.07, 300.54 (100), 292.68	Rutin	610.5	C₂₇H₃₀O₁₆