Baicalin Mitigates the Neuroinflammation through the TLR4/MyD88/NF-κB and MAPK Pathways in LPS-Stimulated BV-2 Microglia

Figure 2

BA inhibits the production and expression of inflammatory molecules in LPS-stimulated BV-2 microglia. The effects of BA on NO production (a), iNOS activity (b), IL-1β (c), and PGE2 (d) production in LPS-stimulated BV-2 cells. BV-2 cells were incubated for 1 h in the presence of the indicated concentrations of BA (2.5-22.5 μM). Then, the cells were stimulated with or without LPS (0.1 μg/mL); the production of NO and iNOS (incubated for 12 h) and IL-1β and PGE2 (incubated for 24 h) was determined by ELISA kit. MiR-155 expression (e) was detected by miRNA real-time PCR kit, BV-2 was pretreated with BA (2.5-22.5 μM) for 1 h followed by LPS stimulation for 12 h, and then, RT-PCR was performed. Results were presented as (). ^### vs. control and , , and vs. LPS-treated cells.

(a)

(b)

(c)

(d)

(e)