[Retracted] A Comprehensive Review of Performance of Next-Generation Sequencing Platforms
Table 8
Advantages and disadvantages of sequencing generations.
Sequencing generation
Advantages
Disadvantages
First generation
High accuracy
High cost
Helps in validating findings of NGS
Low throughput
Second generation
High throughput
Short read length
Low cost
Difficult sample preparation
Have clinical applications
PCR amplification
Short run time
Long run time
Third generation
No PCR amplification
High sequencing error rate 10–15% in the PacBio and 5–20% in the ONT Fresh DNA requires for ensuring quality of ultralong reads Database systems and algorithms/tools are rare for analyzing 3rd and 4th GS data
Require less starting material
Longer read lengths
Very low cost
Low error rate during library preparation
Fourth generation
Advantages of 3rdGS+
Ultrafast: scan of whole genome in 15 minutes
Spatial distribution of the sequencing reads over the sample can be seen
