Research Article
A Novel In Vitro Method to Assess the Microbial Barrier Function of Tissue Adhesives Using Bioluminescence Imaging Technique
Figure 1
Experiment design of pretest 1: (a) group assignment; (b) representative pictures of the bioluminescence imaging from above, bottom site, and cross section indicating the light intensity with the red color as the most intense and the blue color as the least intense signal and the set region of interest (ROI). (c) Experimental design D-1: start of the E. coli Lux culture overnight 1 day before of experiment; D0: testing of E. coli Lux culture by optical density (OD600nm), titration assay, and CFU/mL measurement and application of E. coli Lux to test and control articles. 1 h, 24 h, 48 h, 72 h: assessment of morphology (bacterial growth), adhesion of PU-glue/sterile filter papers, and bioluminescence imaging. (d) Image of E. coli Lux titration (1 : 2 dilution series, row A/B-1 to A/B-10). Values in A/B-12 represent measurements of the control (media only). (e) Linear regression graphs for E. coli Lux titration and bioluminescence results and calculated values.
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