Review Article

Phytochemistry and Biological Properties of Salvia verbenaca L.: A Comprehensive Review

Table 4

Antioxidant activity of S. verbenaca.

Part usedExtractsMethods usedKey resultsRef.

Not specifiedMethanolic extract (80%)
Ethanolic extract (80%)
DPPH assay
β-Carotene/linoleic acids
The 80% methanol extract prepared by maceration was highly active
The 80% of ethanol extract was the most active
[41]
Aerial parts (stems and leaves)Hydromethanolic extractOxygen consumptionA strong inhibition of oxygen consumption (92%)[44]
Conjugated diene formation (CD)A strong inhibition of CD formation of LDL peroxidation (92%)
Thiobarbituric acid reactive substance (TBARS) formationA strong inhibition of TBARS formation of linolenic acid oxidation (93%)
Not specifiedMethanolic extractDPPH assay
β-Carotene–linoleic acid method

[38]
Aerial partsMethanolic extractDPPH assay[42]
ABTS assayIC50 at
TEAC at
IC50 at
TEAC at
TEAC at
TEAC at
Aerial partsMethanolic extract from postdistilled plantDPPH methodSers:
Touiref:
Beja:
Chott Meriem:
Tunis:
Rass Zebib:
Bou Arada:
Bir Mroua:
Hencha:
Enfidha:
[6]
ABTS methodHencha: trolox/mg
Enfidha: trolox/mg
Bir Mroua: trolox/mg
Rass Zebib: trolox/mg
Bou Arada: trolox/mg
Tunis: trolox/mg
Chott Meriem: trolox/mg
Sers: trolox/mg
Beja: trolox/mg
Touiref: trolox/mg
FRAPBeja: Fe+2/mg
Sers: Fe+2/mg
Touiref: Fe+2/mg
Chott Meriem: Fe+2/mg
Tunis: Fe+2/mg
Bou Arada: Fe+2/mg
Rass Zebib: Fe+2/mg
Bir Mroua: Fe+2/mg
Hencha: Fe+2/mg
Enfidha: Fe+2/mg
Aerial partsCrude extractDPPH method[14]
Aerial partsMethanolic extractDPPH
FRAP

High reducing power
[43]
Not specifiedMethanolic extractDPPH method[12]
Aerial partsMethanolic extract subfractions:
Crude extract (CrE)
Chloroform extract (ChE)
Ethyl acetate extract (EAE)
Aqueous extract (AqE)
DPPH methodEAE:
CrE:
ChE:
AqE:
[21]
Reducing power methodEAE: EC50: 0.0047 mg/mL
CrE: EC50:  mg/mL
AqE: EC50: 0.0455 mg/mL
Metal chelating methodAqE and CrE reported the highest activity
EAE chelation did not exceed 20%
Aerial partsMethanolic extracts (85%) (ME)
Decoction extract (distilled water) (DE)
DPPH methodME: IC50: μg/mL
DE: IC50: μg/mL
[13]
ABTS methodME: IC50: μg/mL
DE: IC50: μg/mL
Alkaline DMSO superoxide radical scavengingME: IC50: μg/mL
DE: IC50: μg/mL
β-Carotene bleachingDE: inhibition:
ME: inhibition:
Reducing power methodDE: EC50: μg/mL
ME: EC50: μg/mL
Metal chelating activity methodME: IC50: μg/mL
DE: IC50: μg/mL
Phenanthroline methodME: IC50: μg/mL
DE: IC50: μg/mL
Cupric reducing antioxidant capacity (CUPRAC)ME: A0.50: μg/mL
DE: A0.50: μg/mL
RootMethanolic extractH2DCF-DA methodSignificant reduction in the intracellular reactive oxygen species (ROS) level for both tested values (1 and 10 μg/mL)[14]