CD96 Downregulation Promotes the Immune Response of CD4 T Cells and Associates with Ankylosing Spondylitis

Figure 2

Flow cytometry. (a) Circling gate scheme of flow cytometry analysis. Gating lymphocytes according to the size and granularity (FSC and SSC). Circle the live immune cells “CD45 live” according to CD45 and 7AAD after obtaining single cells. In the gate of “CD45 live,” NK cells (CD3^-CD56⁺) and conventional CD3 T cells (CD3⁺ CD56^-) were sorted out. CD3 T cell (CD3⁺ CD56^-) gate was divided into CD4 T and CD8 T cell subsets according to the expression of CD4 and CD8. (b) Histogram of the expression of CD112R and CD96 between samples and isotype. Compared to the isotype, the expression of CD112R increased slightly, but that of CD96 increased significantly. (c) Frequencies of each lymphocyte subset expressing CD96. The frequencies of CD96-positive CD45 live cells, CD3 T cells, and CD4 T cells in AS patients were lower than those in healthy controls, but no significant differences were observed on CD8 T and NK^- cells. (d) Comparison of the fluorescence intensities of CD96 expressed on lymphocyte subsets. The fluorescence intensities of CD96 expressed on CD45 live cells, CD3 T cells, and CD4⁺T cells were lower in AS patients than in healthy controls, but no significant differences were observed on CD8 T and NK cells ().

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