Combination Treatment of Omega-3 Fatty Acids and Vitamin C Exhibited Promising Therapeutic Effect against Oxidative Impairment of the Liver in Methotrexate-Intoxicated Mice

Table 2

Assessment of acute liver injury according to ranking tissue lesion severity from 0 to 3.

Score components		GR. A	GR. B	GR. C	GR. D	GR. E	GR. F	GR. G
Cellular necrosis	Score	0	+++	+	++	0	0	0
	Extent	No	>60%	<20%	>30%	No	No	No
Inflammatory cell infiltration	Score	0	+++	+	++	0	0	0
	Extent	No	>60%	<20%	>30%	No	No	No
Sinusoid dilatation	Score	0	0	+	0	+	0	+/-
	Extent	No	No	<20%	No	<20%	No	<5%
Fat droplet accumulation	Score	0	0	0	0	+	0	0
	Extent	No	No	No	No	<20%	No	No
Degenerative changes of hepatocyte cells	Score	0	+++	0	++	0	+	+/-
	Extent	No	>60%	No	>30%	No	<20%	<5%

No histopathological changes score (0), very mild histopathological changes in <5% of fields score (+/-), mild histopathological changes in <20% of fields score (+), moderate histopathological changes in 20 to 60% of fields score (++), and severe histopathological changes in >60% of fields score (+++). GR. A = control group; GR. B = MTX-injected group; GR. C and D = omega-3 pretreatment group in graded doses (100 mg and 200 mg); GR. E and F = vitamin C pretreatment group in graded doses (100 mg and 200 mg); GR. G = concomitant pretreated group with omega-3 (100 mg/kg) and vitamin C (100 mg/kg) for 10 days (each group contain 7 mice).