Maackiain Prevents Amyloid-Beta–Induced Cellular Injury via Priming PKC-Nrf2 Pathway

Figure 1

Maackiain prevented Aβ₄₂-induced PC12 cell injury. (a) PC12 cells were stimulated by different doses of Aβ₄₂ (0, 0.1, 10, and 100 μM) for 24 hour or 10 μM Aβ₄₂ at different timepoints (0, 6, 12, 24, and 48 hours). CCK-8 assay was conducted to determine the cell viability in PC12 cells. (b) PC12 cells were stimulated by different doses of Maackiain (10, 20, and 50 μM) for 6 hours and then exposed to Aβ₄₂ (10 μM) for additional 24 hours. CCK-8 (b) and LDH activity (c) assay were performed to detect the viability and injury severity in PC12 cells. TUNEL staining (d) and caspase-3 activity assay (e) were performed to assess the cell apoptosis. and .

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