Maackiain Prevents Amyloid-Beta–Induced Cellular Injury via Priming PKC-Nrf2 Pathway

<div>Maackiain abolished oxidative stress in PC12 cells stimulated with A<i>β</i><sub>42</sub>. PC12 cells were stimulated by different doses of Maackiain (10, 20, and 50 <i>μ</i>M) for 6 hours and then exposed to 10 <i>μ</i>M A<i>β</i><sub>42</sub> for additional 24 hours, followed by determination of intracellular ROS level by DCFH-DA assay (a), mitochondrial membrane potential with rhodamine 123 coupled with flow cytometry (b), SOD activity (c), and MDA levels (d). <svg height="9.2729pt" id="M8" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -8.6359 52.4989 9.2729" width="52.4989pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M471 153C471 170 463 194 452 212C400 220 373 229 322 255C373 281 400 290 452 298C463 316 471 339 471 357C456 366 431 371 410 370C377 329 356 310 308 279C311 336 317 364 336 413C326 432 310 451 294 459C279 451 262 432 252 413C271 364 277 336 280 279C232 310 211 329 178 370C157 371 132 367 117 357C117 340 125 316 136 298C188 290 215 281 266 255C215 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BioMed Research International

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Figure 2

Figure 2: Maackiain Prevents Amyloid-Beta–Induced Cellular Injury via Priming PKC-Nrf2 Pathway 