Exendin-4 Attenuates Hepatic Steatosis by Promoting the Autophagy-Lysosomal Pathway

Figure 3

Exendin-4 enhances autophagic flux. (a) HepG2 cells were treated with oleic acid (OA; 250 μM) or palmitic acid (PA; 250 μM) for 24 h. Then, cells were treated with or without exendin-4 (200 nM) for another 24 h. Cell lysates were collected and immunoblotted with the indicated antibodies. (b) Cells were treated as described in (a). After treatment, cells were stained for LC3 and LAMP1 by immunofluorescence. Scale bar: 50 μm. Yellow arrows indicate LC3 autophagic vacuoles. White arrows represent colocalized lysosome (LAMP1) with LC3 puncta. All experiments were repeated three times with similar results.

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