Alpha-Amylase Inhibits Cell Proliferation and Glucose Uptake in Human Neuroblastoma Cell Lines

<div>Images of the expression of GLUT1 and GLUT2 in control SH-SY5Y cells and cells treated with <i>α</i>-amylase for 72 h. <i>α</i>-Amylase was added to the final concentration of 2 U/l (control received 0.9% NaCl) at 24 h after seeding. GLUT1: (a–d) control; (e–h) <i>α</i>-amylase. GLUT2: (i–l) control; (m–p) <i>α</i>-amylase. NB cells were fixed in 3.7% formaldehyde and stained to visualize cell nuclei (DAPI, blue), actin filaments (Alexa Flour™ 594, red), and GLUT1 or GLUT2 (Alexa Flour™ 488, green). The scale bar is 50 <i>μ</i>m.</div>

BioMed Research International

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Figure 5

Figure 5: Alpha-Amylase Inhibits Cell Proliferation and Glucose Uptake in Human Neuroblastoma Cell Lines 