Evaluation of Zuo-Gui Yin Decoction Effects on Six CYP450 Enzymes in Rats Using a Cocktail Method by UPLC-MS/MS

Table 4

The method validation of six probe drugs in rat plasma sample.

Compounds	RSD (%) of probe drugs
	Lower limit	Interday precision	Intraday precision	Accuracy	Matrix effect
					LQC	MQC	HQC
Phenacetin	4.92	5.73	10.11	5.64	6.73	2.40	1.17
		4.83	8.83	9.26
		2.22	4.17	2.50
		3.45	2.99	0.70
Bupropion	5.60	2.62	4.68	4.42	4.33	1.91	4.50
		5.75	1.11	3.22
		4.53	6.32	3.06
		3.00	2.32	2.05
Amodiaquine	8.88	11.80	9.45	2.14	4.73	1.94	2.85
		4.97	5.23	5.10
		5.44	8.59	4.49
		4.38	2.63	1.51
Omeprazole	5.60	6.05	8.11	5.00	3.02	0.95	2.46
		8.36	1.62	3.57
		4.20	1.73	2.74
		2.49	5.97	1.40
Dextromethorphan	4.43	14.43	7.14	13.61	4.15	7.41	2.31
		12.51	9.78	7.46
		3.01	7.13	5.30
		2.74	2.55	1.30
Midazolam	11.49	7.83	10.36	9.31	4.88	3.81	3.64
		9.83	11.47	10.51
		2.53	1.15	1.79
		4.01	3.60	1.08