Preliminary Analysis of the Glycolipid Profile in Secondary Brain Tumors

Table 2

Assignment of ions obtained by MALDI TOF MS¹ screening of brain tumoral lipid mixture.

No.	m/z	Ion	Structure
1	390.948	[M-H]^-	FA 26 : 2 (17,20) or FA 26 : 2 (5,9)
2	521.072	[M-H]^-	Cer (d18:1/15 : 0)↔Cer 33 : 1; O2
3	536.072	[M-H]^-	Cer (d18:1/16 : 0) or Cer (d18:0/16 : 1) or Cer (d20 : 1/14 : 0) ↔ Cer 34 : 1; O2
4	554.045	[M-H]^-	Cer (t18:0/16 : 0)↔Cer 34 : 0; O3
5	568.019	[M-H]^-	Cer (t18:0/17 : 0)↔Cer 35 : 0; O3
6	590.004	[M-H]^-	Cer (t18:0/19 : 3)↔Cer 37 : 3; O3
7	606.290	[M-H]^-	Cer (d18:1/21 : 0) or Cer (d16:1/23 : 0)↔Cer 39 : 1; O2
8	618.100	[M-H]^-	Cer (d18:2/22 : 0)↔Cer 40 : 2; O2 or Cer (d18:0/16 : 0)–1-P↔CerP 34 : 0; O2
9	640.134	[M-H]^-	Cer (t18:0/h21 : 0)↔Cer 39 : 0; O4
10	654.127	[M-H]^-	Cer (t18:0/24 : 6)↔Cer 42 : 6; O3
11	658.152	[M-H]^-	Cer (t18:0/24 : 4)↔Cer 42 : 4; O3
12	666.217	[M-H]^-	Cer (t18:0/24 : 0) or Cer (t20:0/22 : 0) or Cer (d20:0/h22 : 0)↔Cer 42 : 0; O3
13	670.121	[M-H]^-	Cer (t18 : 0/25 : 5)↔Cer 43 : 5; O3
14	672.144	[M-H]^-	Cer (t18:0/25 : 4)↔Cer 43 : 4; O3
15	692.135	[M-H]^-	Cer (d18:0/27 : 0)↔Cer 45 : 0; O2 or Cer (d18:1/h26 : 0) ↔; Cer 43 : 1; O3
16	714.123	[M-H]^-	Hex-Cer (t18:2/h16 : 0)
17	728.120	[M-H]^-	SM(d18:1/18 : 1) or SM(d18 : 0/18 : 2) or SM(d18:2/18 : 0)
18	730.094	[M-H]^-	Hex-Cer (t18 : 0/17 : 0)
19	744.093	[M-H]^-	Hex-Cer (t18:0/18:0) or hex-Cer (d20:0/h16 : 0)
20		[M-H]^-	Hex-Cer(d18:1/21 : 3)
21	766.078	[M-H]^-	Sulfatides: HSO3-3Galβ-Cer (d18 : 1/h14:0) or; Hex-Cer (d18:2/21:0)
22	776.036	[M-H]^-	Cer (d18:1/h32 : 0)↔Cer 50 : 1; O3
23	792.620		Hex-Cer (d18:1/h20:0)
24	798.594	[M-H]^-	Hex-Cer (d18:1/24 : 6)
25	806.184	[M-H]^-	Hex-Cer (t18:0/23 : 4)
26	864.709	[M-H]^-	Hex-Cer (t18:0/24 : 1); O-ac-lac-Cer (d18:1/12 : 0)
27		[M-H]^-	Lac-Cer (d18:1/18 : 1)
28	914.250	[M-H]^-	Lac-Cer (d18:1/20 : 1)
29		[M-H]^-	GM4 (d16 : 0/13 : 0)
30	953.470		GM4 (d18:0/10 : 0)
31	974.073	[M-H]^-	Sulfatides: HSO3-3Galβ-Cer (d18:1/30 : 0); Or lac-Cer (d18:0/24 : 0)
32	1007.031	[M-H]^-	GA2 (d18:1/12 : 0)
33	1077.730	[M-H]^-	GA2 (t18:1/16:1)
34	1108.151	[M-H]^-	GA2 (d18:1/h18:0)
35	1129.836	[M-H]^-	GM4(d18:1/26 : 0)
36	1181.756	[M-H]^-	GM3 (d18:0/18 : 0)
37	1215.049	[M-H]^-	GM4(d18:0/32:0)
38	1407.710	[M-H]^-	KDN-GM1(t16:1/12 : 0)
39	1426.274	[M-H]^-	GalNAcβ1-4(NeuGcα2-3)Galβ1-4Glcβ-Cer(d18:1/20 : 0)
40	1466.801		GD3 (d18:0/16 : 0)
41	1467.243	[M-H]^-	GM2 (d18:1/24 : 0)
42	1516.941	[M-H]^-	GM1 (d18:1/16 : 0)
43	1544.947	[M-H]^-	GM1 (d18:1/18:0)
44.	1591.939	[M-H]^-	Ganglio:Galβ1-4GalNAcβ1-3Galβ1-3GalNAcβ1-4Galβ1-4Glcβ-Cer(d18:1/16:0) (Ganglio series); Lacto:Galβ1-3GlcNAcβ1-3Galβ1-3GlcNAcβ1-3Galβ1-4Glcβ-Cer (d18:1/16 : 0) (lacto series); Neolacto: Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ-Cer (d18:1/16 : 0)(Neolacto series); Globo: GalNAcα1-3Galβ1-3GalNAcβ1-3Galα1-4Galβ1-4Glcβ-Cer (d18:1/16 : 0)(Globo series); Isoglobo: Galα1-3(GalNAcβ1-4Galβ1-4GlcNAcβ1-6); Galβ1-4Glcβ-Cer (d18:1/16 : 0)(Isoglobo series)
45.	1631.857	[M-H]^-	Type I A antigen: GalNAcα1-3(Fucα1-2)Galβ1-3GlcNAcβ1-3Galβ1-4Glcβ-Cer(d18:1/20 : 0)(lacto series); Type II A antigen: GalNAcβ1-3(Fucα1-2)Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ-Cer(d18:1/20 : 0); Or GalNAcα1-3(Fucα1-2)Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ-Cer(d18:1/20 : 0)(Neolacto series); Globo:Fucα2-3GlcNAcβ1-6GalNAcβ1-3Galα1-4Galβ1-4Glcβ-Cer(d18:1/20 : 0) (Globo series); Isoglobo: Fucα1-3GlcNAcβ1-3Galα1-3(GalNAcβ1-4); Galβ1-4Glcβ-Cer(d18:1/20 : 0)(Isoglobo series)
46.	1758.206	[M-H]^-	GD2(d18:1/24 : 0)
47.	1759.163	[M-H]^-	KDNα2-3Galβ1-4(Fucα1-3)GlcNAcβ1-3Galβ1-4Glcβ-Cer(d18:1/26 : 1)
48.	1873.273	[M-H]^-	GT3(d18:1/26 : 0)
49.	2092.106	[M-H]^-	Fuc-GD1b(d18:1/26 : 1) or; Disialyl lea (d18:1/26 : 1): NeuAcα2-3Galβ1-3 (NeuAcα2-6) (Fucα1-4) GlcNAcβ1-3Galβ1-4Glcβ-Cer(d18:1/26 : 1)
50.	2194.225	[M-H]^-	GalNAcβ1-4(NeuAcα2-3)Galβ1-3GlcNAcβ1-3(Galβ1-3GalNAcβ1-4)Galβ1-4Glcβ-Cer(d18:1/24 : 1)
51.	2286.826	[M-H]^-	sLea -Lex: NeuAcα2-3Galβ1-3(Fucα1-4)GlcNAcβ1-3(Galβ1-4(Fucα1-3)GlcNAcβ1-6)Galβ1-4Glcβ-Cer(d18:1/24 : 0) or; sLea-x: NeuAcα2-3Galβ1-3(Fucα1-4)GlcNAcβ1-3Galβ1-4(Fucα1-3)GlcNAcβ1-3Galβ1-4Glcβ-Cer(d18:1/24 : 0)

FA: fatty acid; Cer: ceramide; Gal:Galactose; Glc:Glucose, Lac: Lactose; Ac: acetyl; SM: sphingomyelins; NeuAc: N-acetylneuraminic acid; KDN: 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid; NeuGc; N-Glycolylneuraminic acid.