Rapid Detection of vanA Resistance Gene from E. faecalis Clinical Isolates Using Duplex Loop-Mediated Isothermal Amplification and Triplex PCR Assay

Figure 1

(a) PCR product electrophoresis of Enterococcus isolates. Lane 1: 100 bp DNA ladder; lane 2: negative control; and lane 3: positive control with 305 bp product for E. faecalis, 195 bp product for vanA, and 109 bp product for Enterococcus. (b) LAMP electrophoresis of amplified Enterococcus isolates. Lane 1: 8: 50 bp DNA ladder; lane 2: single reaction for detection of E. faecalis; lane 3: the duplex-LAMP for detection of E. faecalis and vanA gene; lane 4: the single reaction for detection of vanA; lane 5: EcoRI digested single reaction for detection of E. faecalis; lane 6: EcoRI digested of duplex-LAMP for detection of E. faecalis and vanA gene; and lane 7: EcoRI digested of single reaction for detection of vanA.

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