Rapid Detection of vanA Resistance Gene from E. faecalis Clinical Isolates Using Duplex Loop-Mediated Isothermal Amplification and Triplex PCR Assay

Figure 3

(a) LAMP primers targeting the VanA (E. faecalis) gene were used in the serial dilutions to determine limit of detection. 1: negative control; 2: positive control; 3: 1 ng; 4: 0.01 ng; 5: 1 pg; 6: 0.01 pg; 7: 1 fg (LOD of E. faecalis); 8: 0.1 fg; 9: 0.01 fg; 10: 1 Ag from purify DNA of E. faecalis. (b) LOD of E. faecalis by PCR assay. 1: 100 bp DNA ladder; 2: positive control (109 bp Enterococcus, 195 bp vanA, and 305 bp E. faecalis); 3: negative control; 4: 10 ng; 5: 1 g; 6 : 0.1 ng; 7: 10 pg (LOD of vanA); 8: 1 pg; 9: 0.1 pg (LOD of E. faecalis); 10: 10 fg (LOD of Enterococcus); 11: 1 fg; 12: 0.1 fg; and 13: 0.01 fg from purify DNA of E. faecalis.

(a)

(b)