Research Article
Rapid Detection of vanA Resistance Gene from E. faecalis Clinical Isolates Using Duplex Loop-Mediated Isothermal Amplification and Triplex PCR Assay
Table 1
List of PCR and LAMP primers that were designed and used in this study.
| | Primer name | Sequence (5 to 3) | Product size | Reference |
| | Enterococcus tuf gene | F: TAC TGA CAA ACC ATT CAT GAT G
R: TTC GTC ACC AAC GCG AAC | 109 bp | This study | | E. feacalis ddl gene | F: CCA CAA GTA CCA TTC GTG C
R: GCG ACA TCT TTC ACC ACT TC | 305 bp | This study | Tn 1546
VanA gene | F: TCG TTG ACA TAC ATC GTT GC
R: TGT CTT GCC GAT TCA ATT GC | 195 bp | This study | | Van A BIP | CCGCAGACCTTTCAGCAGAG-GAATTC-GAGCGCTTTATATATTTTTTTTGCC | | This study | | Van A FIP | GAACGGTTATAACTGCGTTTTCAG-GAATTC-ATCTTTCGTATTCATCAGGAAG | | This study | | Van A B3 | GGGCTAGACCTCTACAGC | | This study | | Van A F3 | AAATCAGGCTGCAGTACG | | This study | | Van A LB | AGCGAGGACGGATACAGGA | | This study | | Van A LF | AGCCTTTTTCCGGCTCGA | | This study | | E. faecalis BIP | CATTCCACAAGTACCATTCGTGC-GAATTC-CCTTCACATTTTTCAAAGACTTC | | This study | | E. faecalis FIP | TCCATTGCGTTAACGCTAGCTA-GAATTC-ATGGAAACCATTAATATGCCTT | | This study | | E. faecalis B3 | AGGTTTAACAAAGACCGGATA | | This study | | E. faecalis F3 | AGATGGAACAATTCAAGGATTC | | This study | | E. faecalis LB | TAAGAAGTGACTGGAAAGGAAATCC | | This study | | E. faecalis LF | GACACCCGCGCCTACATAA | | This study |
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