Research Article
Rapid Detection of vanA Resistance Gene from E. faecalis Clinical Isolates Using Duplex Loop-Mediated Isothermal Amplification and Triplex PCR Assay
Table 3
Clinical specificity and sensitivity of the PCR, LAMP, and API.
| | PCR | LAMP | API |
| | Sensitivity | =94.19% | 95% CI: 86.94% to 98.06% | =94.19% | 95% CI: 86.94% to 98.06% | =93.57% | 95% CI: 86.00% to 97.92% | | Specificity | =72.73% | 95% CI: 49.78% to 89.20% | =72.73% | 95% CI: 49.78% to 89.20% | =53.57% | 95% CI: 33.88% to 72.47% | | Positive likelihood ratio | =3.45 | 95% CI: 1.74% to 6.85% | =3.45 | 95% CI: 1.74% to 6.85% | =2.02 | 95% CI: 1.35% to 3.02% | | Negative likelihood ratio | =0.08 | 95% CI: 0.03% to 0.19% | =0.08 | 95% CI: 0.03% to 0.19% | =0.12 | 95% CI: 0.05% to 0.29% | | Disease prevalence | =79.63% () | 95% CI: 70.80% to 86.77% | =79.63% () | 95% CI: 70.80% to 89.20% | =74.07% () | 95% CI: 64.75% to 82.03% | | Positive predictive value | =93.10% () | 95% CI: 85.58% to 97.41% | =93.10% () | 95% CI: 85.58% to 97.41% | =85.23% () | 95% CI: 76.06% to 91.89% | | Negative predictive value | =76.19% () | 95% CI: 52.83% to 91.69% | =76.19% () | 95% CI: 52.83% to 91.69% | =75.00% () | 95% CI: 50.89% to 91.25% | | KAPA | 0.8981 | 0.8981 | 0.8333 |
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