Experimental Study of Potential CD8⁺ Trivalent Synthetic Peptides for Liver Cancer Vaccine Development Using Sprague Dawley Rat Models

Table 2

Physicochemical properties of the individual epitopes and trivalent construct.

UniProt ID	Peptide sequence	SVM score	Toxicity prediction	Hydropathicity	Charge	Half-life	Instability index
ALBU_HUMAN	LLECADDRADLAKY	-0.36	Nontoxin	-0.31	-2	5.5 hours	23.16
BTNL2_HUMAN	VSEHRIQDKDGLFY	-1.17	Nontoxin	-0.91	-0.5	10 hours	48
GPC3_HUMAN	EYILSLEELVNGMY	-0.57	Nontoxin	0.3	-3	1 hour	52
TRIVALENT	VSEHRIQDKDGLFYAAYLLECADDRADLAKYAAYEYILSLEELVNGMY	-0.84	Nontoxin	-0.221	-6.5	100 hours	34.49