Upregulated Fibulin-1 Increased Endometrial Stromal Cell Viability and Migration by Repressing EFEMP1-Dependent Ferroptosis in Endometriosis

Figure 4

FBLN1 increased the stability of EFEMP1 protein. (a) STRING tool (https://string-db.org/) was applied to predict the proteins that may interact with FBLN1. (b) EESCs were treated with siFBLN1, and then, qPCR analysis was carried out to assess the mRNA level of EFEMP1 in EESCs. (c, d) EESCs were treated with siFBLN1 (or N.C), and then, western blot analysis was carried out to assess the protein level of EFEMP1 in EESCs and NESCs. (e) EESCs were treated with siFBLN1 (or N.C) in the presence of CHX, and then, western blot analysis was carried out to assess the EFEMP1 protein level at 0, 2, 4, and 8 h. (f) Relative quantitative analysis of western blot showed in (e). (g, h) NESCs were treated with pcDNA-FBLN1 (or control) in the presence of CHX, and then, western blot analysis was carried out to assess the EFEMP1 protein level at 0, 2, 4, and 8 h.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)