Effects of Etanercept on TNF-α Inhibition in Rats with Adenine-Induced Chronic Kidney Disease

Figure 4

Kidney histology. (a) Hematoxylin and eosin stain. Normal kidney architecture was observed in control animals. Interstitial inflammatory infiltrates, crystalline tubulointerstitial deposits, and tubular atrophy are showed in the Ad groups. A partial decrease of interstitial inflammatory infiltrates and tubular atrophy was observed after ETA treatment. (b) Thy-1/DAPI immunostain. The Ad-CKD group exhibited less Thy-1 expression (tubular atrophy), whereas ETA treatment partially maintains the expression of Thy-1 (3 cortical fields per animal were analyzed) (40x). Scale bar is 20 μm. (c) Masson’s trichromic staining showed extracellular matrix accumulation in the Ad groups. ETA does not reduce kidney fibrosis at 4 weeks. All are representative photomicrographs of kidney sections in an approximation of 40x.