Research Article
Silencing the ADAM9 Gene through CRISPR/Cas9 Protects Mice from Alcohol-Induced Acute Liver Injury
Figure 10
In vitro analysis of the effects of alcohol on the expression levels of ADAM9, HSP27, HSP70, PCNA, Bax, Bcl-2, caspase-3, VEGF, and p-STAT3 in hepatic L02 cells. (a) Western blotting results. The expression levels of (b) ADAM9, (c) HSP27, (d) HSP70, (e) PCNA, (f) Bax, (g) Bcl-2, (h) caspase-3, (i) VEGF, and (j) p-STAT3 quantified using Gel-Pro Analyzer 4.0 software (Media Cybernetics Inc.). Band intensities normalized to β-actin. AU = arbitrary unit. and : significant differences between the normal+ADAM9-sgRNA3, saline+alcohol, ADAM9-sgRNA+alcohol, and normal group. ## and #: significant differences between the normal+ADAM9-sgRNA3, ADAM9-sgRNA+alcohol, and saline+alcohol groups. △△ and △: significant difference between the ADAM9-sgRNA+alcohol group and the normal+ADAM9-sgRNA3 group.
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