Silencing the ADAM9 Gene through CRISPR/Cas9 Protects Mice from Alcohol-Induced Acute Liver Injury

<div>Electrophoresis profiles of transformant positive clones obtained by PCR. The band of approximately 100 bp was a positive clone, and the leftmost lane contained the DNA marker: 100, 250, 500, 750, 1000, 1500, 2000, 3000, and 5000 bp. Lanes 2–6: pYSY-CMV-Cas9-U6-ADAM9-gRNA1-EFla-Puromycin clones; lanes 7–11: pYSY-CMV-Cas9-U6-ADAM9-gRNA2-EFla-Puromycin clones; lanes 12–15: pYSY-CMV-Cas9-U6-ADAM9-gRNA3-EFla-Puromycin clones.</div>

BioMed Research International

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Figure 2

Figure 2: Silencing the ADAM9 Gene through CRISPR/Cas9 Protects Mice from Alcohol-Induced Acute Liver Injury 