Mitochondrial Haplogroup Classification of Ancient DNA Samples Using Haplotracker

Figure 2

Design of a representative primer pair for variant detection via HRM real-time PCR. The HRM real-time PCR primers to detect the nucleotide changes at the variation site of haplogroup G1a1 (A-to-G transition at nucleotide position 15860 of rCRS) target sites very close to the variation site. The amplicons were as small as 46 bp. The small size conferred large differences to the amplicons and thereby enhanced the discrimination of single-nucleotide differences. Arrows indicate primer positions. Numbers mark the nucleotide position of rCRS. The base in bold represents a variation site for haplogroup G1a1. HRM: high-resolution melting.