Bioactivity of Exosomes Derived from Trained Natural Killer Cells versus Non-Trained One: More Functional and Antitumor Activity

Figure 2

Exosome characterization. The exosome morphology as a spherical lipid bilayer vesicle for non-trained (a) and trained Exos (b) was confirmed by TEM. The size distribution and Zeta potential were obtained as 142.5 nm and -2.3 mV for non-trained Exos (c) and as 136.0 nm and -3.1 mV for trained Exos (d). The positive expression of CD9 and CD63, the general Exos markers, and non-expression of calnexin were analyzed by western blot (e). The expression of NK-Exos specific proteins (CD56) was analyzed by using flow cytometry for non-trained Exos (f) and trained Exos (g).

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