Bioactivity of Exosomes Derived from Trained Natural Killer Cells versus Non-Trained One: More Functional and Antitumor Activity

Figure 3

Cytotoxicity of trained Exos compared to non-trained Exos. K562 cells were seeded in a 24-well plate, and 25 μg of PKH26 labeled exosomes was added to each well. The red fluorescent was visualized by an inverted fluorescence microscope. The fluorescent intensity due to the presence of red PKH26 color demonstrated the existence of exosomes (a). IC50 values were assessed 5.29 μg/well for non-trained Exos (b) and 4.13 μ/well for trained Exos (c). The K562 cells were divided into three groups: untreated, treated with 5.29 μg/well of non-trained Exos, and treated with 5.29 μg/well of trained Exos. The cell viability was confirmed by MTT after 48 hours (d). Data are presented as means ± SD of three separate experiments,  =3, vs control groups.

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