Saikosaponin D Inducing Apoptosis and Autophagy through the Activation of Endoplasmic Reticulum Stress in Glioblastoma

Figure 5

SSD-induced cell autophagy of GBM cell lines. Activated the expression of autophagy-related proteins LC3 and Beclin 1 in RG-2, U87-MG, U251, and LN-428 GBM cell lines treated by SSD. (a) ICC staining (40× magnification). (b) Western blotting. β-Actin was used as qualitative and quantitative control. Ratio, the ratio between the levels of the target molecules and that of β-actin; with statistical significance (, , , and vs. CON group). The error bars, the deviation.

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