Identification of Key Genes Associated with Endothelial Cell Dysfunction in Atherosclerosis Using Multiple Bioinformatics Tools

Figure 7

(a) Some results of horizontal electrophoresis of RNA samples from clinical specimens are shown. (b) The Q-PCR results of key genes; CTTN, ITGB1, and RAB5A in atherosclerotic samples were significantly lower than those in the normal group (). On the contrary, MMP9 in atherosclerotic samples was higher than that in the control group (). (c) Establishment of the injured HCAEC model. In the normal group, the HCAECs were uniform and regular, spindle-shaped, and closely connected with each other. In the injured group, the HCAEC morphology was nonuniform, the connection between cells was not very close, and the arrangement was irregular. (d) The LDH activity assays showed that compared to the normal group, LDH activity was significantly increased in the ox-LDL-injured group (). The above results show that the cell model is successful.

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