Identification of Key Genes Associated with Endothelial Cell Dysfunction in Atherosclerosis Using Multiple Bioinformatics Tools

Figure 9

(a) The endothelial cell apoptosis rates in the HCAEC, HCAEC-RAB5A overexpression, HCAEC-RAB5A shRNA, and HCAEC-NC groups were 5.18%, 2.75%, 10.3%, and 4.39%, respectively. (b) Column analysis chart of the apoptosis rate in each group. , compared to the HCAEC group; ^#, compared to the HCAEC-RAB5A overexpression group. (c) The cell proliferation of each group was compared at each time point (24 h, 48 h, 72 h, and 96 h). , HCAEC-RAB5A overexpression compared to HCAEC group; , HCAEC-RAB5A shRNA compared to HCAEC group. (d, e) The cell cycle analysis for all groups. (d) The cell cycle analysis results are shown in (d). The area under the red curve represents the G1 phase of the cell cycle. The area under the curve filled with slashes represents the S phase of the cell cycle. (e) Column analysis chart of the cell cycle in all groups. (f, g) Apoptosis-related protein expressions for all groups. (f) The expression levels of Bax, caspase-3, and Bcl-2 proteins in each group. (g) Relative quantitative comparison of western blot analyses.

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