Computational Clues of Immunogenic Hotspots in Plasmodium falciparum Erythrocytic Stage Vaccine Candidate Antigens: In Silico Approach

Table 1

Prediction of antigenicity, allergenicity, solubility, stability, and physicochemical properties of 14 P. falciparum erythrocytic stage proteins.

Server/Parameter	AMA1	CyRPA	Eba175	MSP1	MSP3	MSP4	MSP6	Ralp1	RESA	Rh5	Ripr	Ron2	Sera5	Sera8
VaxiJen score	0.5639	0.5732	0.6903	0.6039	0.8309	1.0076	0.6790	0.6627	0.5626	0.4882	0.4980	0.5071	0.7150	0.6148
AllergenFP	No	No	No	No	No	No	No	No	Yes	No	No	No	Yes	Yes
AllerTOP	Yes	Yes	No	No	Yes	No	No	No	Yes	No	Yes	No	No	No
Protein-Sol	0.415	0.545	0.505	0.523	0.806	0.894	0.778	0.613	0.714	0.503	0.445	0.400	0.501	0.302
No. of AA	622	362	1435	1639	394	272	427	677	1073	526	1086	1369	997	690
MW	72009.78	42775.68	167390.05	187618.81	45134.89	30548.52	48448.28	79640.22	104906.84	62995.86	125892.52	158975.66	111768.15	80850.47
pI	5.55	5.39	5.62	5.98	4.55	4.57	4.57	5.9	4.46	8.62	6.36	9.37	5.26	8.46
+ residues (Arg+Lys)	76	43	221	234	58	35	47	105	109	83	127	202	109	96
- residues (Asp+Glu)	96	54	258	251	104	65	91	115	243	76	136	151	141	86
Half-life (mammalian reticulocytes)	30 hours	30 hours	30 hours	30 hours	30 hours	30 hours	30 hours	1.1 hours	30 hours	30 hours	30 hours	30 hours	30 hours	30 hours
Instability index	37.10 (stable)	39.20 (stable)	42.42 (unstable)	39.18 (stable)	52.98 (unstable)	43.52 (unstable)	54.73 (unstable)	48.53 (unstable)	51.86 (unstable)	35.04 (stable)	42.59 (unstable)	45.52 (unstable)	37.89 (stable)	36.08 (stable)
Aliphatic index	62.88	88.23	62.67	85.55	57.82	67.65	63.47	64.02	74.04	83.38	70.87	79.39	65.75	69.04
GRAVY	-0.802	-0.330	-1.072	-0.691	-1.354	-0.969	-1.257	-1.302	-0.930	-0.831	-0.607	-0.523	-0.706	-0.757
SignalP	0.9996 (yes)	0.9998 (yes)	0.637 (yes)	0.9987 (yes)	0.693 (yes)	0.266 (no)	0.3255 (no)	0.0062 (no)	0.0001 (no)	0.1967 (no)	0.9997 (yes)	0 (no)	0.9996 (yes)	0.9996 (yes)
TMHMM	1	—	—	—	—	—	—	—	1	—	—	—	—	—
Subcellular localization	CM: 0.7860; ER: 0.6861	EX: 0.8793	CM: 0.5714	CM: 0.7836	EX: 0.8742; CM: 0.5912	CM: 0.8955; EX: 0.6376	CM: 0.7187	CM: 0.4710	ER: 0.3461	CM: 0.6154	EX: 0.6184	Mit: 0.5620	EX: 0.7846; CM: 0.6174	EX: 0.8235