BioMed Research International / 2022 / Article / Tab 4 / Research Article
Computational Clues of Immunogenic Hotspots in Plasmodium falciparum Erythrocytic Stage Vaccine Candidate Antigens: In Silico Approach Table 4 Prediction of shared continuous B-cell epitopes in 14 selected erythrocytic stage P. falciparum antigens with subsequent screening regarding antigenicity, allergenicity, and water solubility.
Protein Shared epitopes VaxiJen antigenicity AllergenFP allergenicity PepCalc water solubility AMA1 DHEGAEPAPQEQNL 0.3882 No Good KMDEPQHYG 1.0432 Yes Good EEYKDEYADI 0.6827 Yes Good LYIAAQENNGPRY 0.3357 No Poor HQEHTYQQED 0.6384 No Good LPTGAFKADRYKS 1.3353 No Good FGRGQNYWEHPYQ -0.2076 No Poor NMIPDNDKNSN 1.1296 No Good TEYMAKYDIE 0.2234 No Good CyRPA SPYKFKDDNKKD 1.0096 No Good DNINNVKNV 0.0707 Yes Good NDLFKETDLTG 1.2358 No Good EEFSNRKKDMT 0.8601 No Good KDNKVLQDVSTL 0.1229 No Good LKDKLLSSYVSLPL 0.9588 No Poor Eba175 HHGNRQDRGGNSGN 2.2151 No Good REDERTLTKEYEDI 0.7318 No Good GPKGNEQKERDDDS 1.5703 No Good SRINNGRNTSS 1.5214 No Good SINNGDDSGSGS 1.3930 No Good SNNNNFNNIPS -0.4858 No Poor EKFNELDKKKYGN -0.0866 No Good YCDCKHTTTLVK 0.9283 No Good EEYNKQAKQYQEYQ 0.8479 No Good NTLHLKDIRNEE 0.4600 No Good GDDSGSGSAT 2.1372 Yes Good NPEDRNSNTLHLK 0.9643 No Good NVQQSGGIVN 1.1086 No Poor KAEEERLSHTDI 0.5411 No Good MSP1 VPTPPAPVNNKTEN 0.6756 No Good NPPPANSGNTPNTL 0.1965 No Poor PPVPVPVPEAK 0.6912 No Good GCFRHLDEREE -0.0243 No Good EQLFEKKIT 0.5568 Yes Good NPSDNSSDSDAK 1.6344 No Good LEQSQPKKPASTHV 0.7679 No Good ILKQYKITKEEESK 0.8440 No Good DKNKKIEEHEKE 1.3583 No Good LNSLNNPKH 0.2690 Yes Good SSNYVVKDPYKFLN 0.2960 No Good VPNSYKQENK 0.6840 No Good LKHNIHVPNSYKQE 0.6190 No Good MSP3 YKKAKEASQDAEKA 0.9912 Yes Good ENVNTPIVGNSM 0.9144 Yes Poor LGWEFGGGVPEHKK 0.4004 Yes Good NNNSQIENEEK 1.3218 No Good MSP4 ASEQGEESHKKE 1.6269 No Good HVGEEEDHNEGEGE 1.6106 Yes Good VVHFFIICTINF -0.0159 Yes Poor KKKTEAIPKKVVQ 0.0166 No Good SDAAEKKDEKEASE 1.1597 Yes Good DDDEDDDTYN 1.4465 No Good MSP6 SDIQATYQFPPTP -0.4445 Yes Poor GTRGIHTYSGES 0.1113 No Good AEENSETNKNPT 1.1044 No Good PPTPGRIINPR 0.8340 Yes Good EEEKKEEEEKKE 1.8461 No Good DKKTEYLLEQI 0.4517 Yes Good Ralp1 IVYNNIQGQG 0.9383 Yes Poor NNIQGQGELLQ 1.0569 Yes Poor GNGDNESSQRVD 1.5302 No Good NVEKKSNMESVNNN 0.5877 Yes Good NANKVPNVAH -0.2316 Yes Poor QSDDITDEQKKY 0.8375 No Good KLDQQGELKNVSVV 0.8150 No Good KDNTFNFHKN 0.5660 No Good NKDNTFNFHKN 0.2417 No Good DENNNTNDKNNC 1.3952 No Good RESA AEMKKRAQK 1.4690 Yes Good PQQEEPVQTVQE 0.7672 No Good PYADSENPIVV 0.6307 Yes Good NVEENVEENVEENV 1.3997 No Good NINSNVDNGN 1.6573 No Good TQANKQELANI 0.1906 No Good YGYDGIKQV -1.0965 Yes Good RWYNKYGYDGIKQV -0.4537 No Good SSSSGVQFTDRCS 0.1913 Yes Good KDFTGTPQIVTLLR -0.0496 No Good NLYGETLPVNPY 1.8128 Yes Poor Rh5 AIKKTKNQEN 0.6552 Yes Good TEEEKDDIKNGKDI 1.2106 No Good SCYNNNFCNTNG -0.0212 No Poor NNKNDDSYRYD 0.7851 No Good IINDKTKIIQD 0.9855 No Good SKNLNKDLSD 0.5424 Yes Good KKLEHPYDINNK -0.5249 No Good SVFNQINDGMLLN 0.2222 No Poor KKINETYDKV -0.4121 Yes Good Ripr TDPHTNSNNI 1.1210 No Good KEKFYKNNLY 1.3379 Yes Good CYKKTFCGVVIP 0.9010 No Poor EIQNEISSHNSNQF 0.4284 No Good SVLCSQNQVCQ 0.0578 No Poor HLISRNSR 0.2658 Yes Good CANDYKMEDGI -0.0054 No Good Ron2 YASKQTSDSDDSDI 1.1198 No Good NDSSEESAKKKLQD 0.6400 No Good WLEFNDNPTNASS 0.5370 No Good KNDTSEKSSQKN 1.1045 No Good PKRTTTFYGERRL 0.1554 No Good DSKGMLARQIFTKG 1.1186 No Good RPKRTTTFYGE 1.4781 No Good RNPGMMAI 0.1053 Yes Poor FSTYMGFDRRSFLP -0.3423 No Poor REQIENFK 0.0655 Yes Good Sera5 SSSSSSSSSSSSSE 1.8405 No Good GASPQGSTG 1.5737 No Poor PEDKDNKGK 2.1879 Yes Good TVSVSQTSTSSEKQ 0.7225 No Good PANGPDSPTVKPP 0.6062 No Good TKDTTENNKVDV 0.9430 No Good LPSNGTTGEQ 1.2054 No Good PMNNKTTKKESKIY 0.8272 No Good KILHNKNEPNSL 0.1980 No Good KTNNAISFESNSGS 0.3549 No Good VNKRGLVLPELNY 0.2452 No Good EDDDEDDYTEY 1.2934 Yes Good YNYVKVGEQCPKVE 0.8915 No Good Sera8 DAYYDNNDDY 0.8582 No Good NMAHNPIDVPLPND 0.6719 No Good FSFEKDSDT 1.1152 Yes Good DKLTFDHDGT -0.0833 No Good NYKDNYENANNH 0.6804 No Good QYDKNSNDYDRN 0.8916 Yes Good SKRKDEYKEPYS 0.8054 No Good IYHGYFKVSFK 0.9309 Yes Poor PITGSECPDN 1.2238 No Good INEENNMEHMKD 0.7345 No Good
