Computational Clues of Immunogenic Hotspots in Plasmodium falciparum Erythrocytic Stage Vaccine Candidate Antigens: In Silico Approach

Table 6

Helper T lymphocyte-specific epitope prediction in 14 selected erythrocytic stage P. falciparum antigens and subsequent screening regarding IFN-γ induction, antigenicity, and allergenicity.

Protein	Allele	HTL epitope	Method	Percentile rank	VaxiJen antigenicity	AllerTOP allergenicity	IFN-γ inducer
							Result	Score
AMA1	HLA-DPA102 : 01/DPB114 : 01	KMKIIIASSAAVAVL	NetMHCIIpan	0.03	0.7487	No	Negative	5
	HLA-DPA102 : 01/DPB114 : 01	DKMKIIIASSAAVAV	NetMHCIIpan	0.06	0.6712	No	Negative	5
	HLA-DRB113 : 02	MKIIIASSAAVAVLA	NetMHCIIpan	0.07	0.7198	No	Negative	7
	HLA-DRB109 : 01	YDKMKIIIASSAAVA	NetMHCIIpan	0.08	0.6072	Yes	Negative	5
	HLA-DQA105 : 01/DQB103 : 01	MKIIIASSAAVAVLA	Consensus (comb.lib./smm/nn)	0.09	0.7198	No	Negative	7
CyRPA	HLA-DRB302 : 02	ELSFIKNNVPCIRDM	NetMHCIIpan	0.01	0.0131	Yes	Negative	-0.2933
	HLA-DQA105 : 01/DQB102 : 01	GRPHIFAYVDVEEII	Consensus (comb.lib./smm/nn)	0.01	-0.2266	No	Positive	0.8518
	HLA-DQA105 : 01/DQB102 : 01	HIFAYVDVEEIIILL	Consensus (comb.lib./smm/nn)	0.01	0.2274	Yes	Negative	12
	HLA-DRB302 : 02	LSFIKNNVPCIRDMF	NetMHCIIpan	0.01	0.1361	Yes	Negative	-0.7208
	HLA-DQA105 : 01/DQB102 : 01	PHIFAYVDVEEIIIL	Consensus (comb.lib./smm/nn)	0.01	0.1163	No	Negative	3
Eba175	HLA-DPA101 : 03/DPB102 : 01	CNISIYFFASFFVLY	Consensus (comb.lib./smm/nn)	0.01	0.9694	Yes	Positive	1
	HLA-DRB112 : 01	IFKFLITNKIYYYFY	Consensus (smm/nn)	0.01	0.1789	Yes	Negative	1
	HLA-DPA101 : 03/DPB102 : 01	ISIYFFASFFVLYFA	Consensus (comb.lib./smm/nn)	0.01	1.5296	No	Positive	1
	HLA-DPA101 : 03/DPB102 : 01	KCNISIYFFASFFVL	Consensus (comb.lib./smm/nn)	0.01	1.1363	No	Positive	0.2194
	HLA-DPA101 : 03/DPB102 : 01	NISIYFFASFFVLYF	Consensus (comb.lib./smm/nn)	0.01	1.5428	No	Positive	1
MSP1	HLA-DPA103 : 01/DPB104 : 02	FLGISFLLILMLILY	Consensus (comb.lib./smm/nn)	0.01	1.3457	No	Negative	64
	HLA-DPA103 : 01/DPB104 : 02	NFLGISFLLILMLIL	Consensus (comb.lib./smm/nn)	0.01	1.1366	No	Negative	60
	HLA-DPA103 : 01/DPB104 : 02	LGISFLLILMLILYS	Consensus (comb.lib./smm/nn)	0.02	0.9541	No	Negative	54
	HLA-DPA103 : 01/DPB104 : 02	GISFLLILMLILYSF	Consensus (comb.lib./smm/nn)	0.03	0.8777	No	Negative	48
	HLA-DPA103 : 01/DPB104 : 02	ISFLLILMLILYSFI	Consensus (comb.lib./smm/nn)	0.03	1.6407	No	Negative	46
MSP3	HLA-DRB302 : 02	HLYIYINNVASKEIV	NetMHCIIpan	0.01	0.2830	Yes	Positive	4
	HLA-DRB302 : 02	LHLYIYINNVASKEI	NetMHCIIpan	0.01	0.6264	Yes	Negative	4
	HLA-DRB302 : 02	LYIYINNVASKEIVK	NetMHCIIpan	0.01	0.1614	Yes	Positive	4
	HLA-DRB302 : 02	YIYINNVASKEIVKK	NetMHCIIpan	0.02	-0.2207	Yes	Positive	1
	HLA-DRB302 : 02	LLHLYIYINNVASKE	NetMHCIIpan	0.09	0.6712	Yes	Negative	-0.3417
MSP4	HLA-DRB101 : 01	CVELLSLASSSLNLI	Consensus (comb.lib./smm/nn)	0.1	-0.0621	No	Positive	1
	HLA-DRB101 : 01	ECVELLSLASSSLNL	Consensus (comb.lib./smm/nn)	0.1	0.1481	Yes	Positive	1
	HLA-DRB101 : 01	VELLSLASSSLNLIF	Consensus (comb.lib./smm/nn)	0.1	0.0252	Yes	Positive	1
	HLA-DRB101 : 01	IECVELLSLASSSLN	Consensus (comb.lib./smm/nn)	0.16	0.2052	Yes	Positive	1
	HLA-DPA101 : 03/DPB102 : 01	LNLIFNSFITIFVVI	Consensus (comb.lib./smm/nn)	0.22	1.0058	No	Negative	8
MSP6	HLA-DPA101 : 03/DPB102 : 01	MNKIYNITFLFILLN	Consensus (comb.lib./smm/nn)	0.14	0.4987	No	Negative	11
	HLA-DPA101 : 03/DPB102 : 01	NKIYNITFLFILLNL	Consensus (comb.lib./smm/nn)	0.14	0.7500	No	Negative	13
	HLA-DRB113 : 02	ILLNLYINENNFIRN	Consensus (smm/nn/sturniolo)	0.22	1.5255	No	Negative	-0.2297
	HLA-DRB113 : 02	LLNLYINENNFIRNE	Consensus (smm/nn/sturniolo)	0.23	1.0849	Yes	Negative	-0.2188
	HLA-DRB113 : 02	LNLYINENNFIRNEL	Consensus (smm/nn/sturniolo)	0.27	1.2208	Yes	Negative	-0.4038
Ralp1	LA-DRB302 : 02	NKKFKLNESMKNHFY	NetMHCIIpan	0.04	0.7270	No	Negative	-0.5034
	HLA-DPA101 : 03/DPB102 : 01	FIIVYCFISSFYLIK	Consensus (comb.lib./smm/nn)	0.06	0.7382	Yes	Negative	4
	LA-DRB302 : 02	KKFKLNESMKNHFYN	NetMHCIIpan	0.06	0.4953	No	Negative	-0.5117
	HLA-DPA101 : 03/DPB102 : 01	IIVYCFISSFYLIKS	Consensus (comb.lib./smm/nn)	0.07	0.5982	Yes	Negative	1
	HLA-DPA101 : 03/DPB102 : 01	FFIIVYCFISSFYLI	Consensus (comb.lib./smm/nn)	0.08	1.1158	Yes	Negative	6
RESA	HLA-DPA101 : 03/DPB102 : 01	IVTLLRFFFEKRLSM	Consensus (comb.lib./smm/nn)	0.24	0.7305	No	Negative	-0.1734
	HLA-DRB107 : 01	ESLRWIFKHVAKTHL	Consensus (comb.lib./smm/nn)	0.28	-0.2477	No	Negative	-0.1925
	HLA-DRB107 : 01	LRWIFKHVAKTHLKK	Consensus (comb.lib./smm/nn)	0.28	0.2922	Yes	Negative	-0.1144
	HLA-DRB107 : 01	RWIFKHVAKTHLKKS	Consensus (comb.lib./smm/nn)	0.28	0.3480	Yes	Negative	-0.2612
	HLA-DRB107 : 01	SLRWIFKHVAKTHLK	Consensus (comb.lib./smm/nn)	0.28	0.0077	Yes	Negative	-0.3372
Rh5	HLA-DRB301 : 01	NDDSYRYDISEEIDD	Consensus (comb.lib./smm/nn)	0.06	0.5481	Yes	Positive	0.1695
	HLA-DRB301 : 01	DDSYRYDISEEIDDK	Consensus (comb.lib./smm/nn)	0.09	0.6564	No	Positive	0.1793
	HLA-DRB301 : 01	DSYRYDISEEIDDKS	Consensus (comb.lib./smm/nn)	0.11	0.7195	Yes	Positive	0.1783
	HLA-DRB301 : 01	KMGSYIYIDTIKFIH	Consensus (comb.lib./smm/nn)	0.11	0.3265	No	Negative	-0.3428
	HLA-DRB301 : 01	KNDDSYRYDISEEID	Consensus (comb.lib./smm/nn)	0.11	0.3230	No	Negative	-0.3932
Ripr	HLA-DRB101 : 01	CQGMYISLRSVHVHT	Consensus (comb.lib./smm/nn)	0.1	0.4897	No	Positive	0.2683
	HLA-DRB101 : 01	GMYISLRSVHVHTHN	Consensus (comb.lib./smm/nn)	0.1	0.6017	No	Positive	0.1345
	HLA-DRB101 : 01	NCQGMYISLRSVHVH	Consensus (comb.lib./smm/nn)	0.1	0.4426	No	Negative	-0.1838
	HLA-DRB101 : 01	QGMYISLRSVHVHTH	Consensus (comb.lib./smm/nn)	0.1	0.7164	No	Positive	0.0831
	HLA-DRB401 : 01	IFFTLLIIILIKKTS	Consensus (comb.lib./smm/nn)	0.12	1.1012	No	Negative	116
Ron2	HLA-DRB101 : 01	GTKSFYSLPTILTAN	Consensus (comb.lib./smm/nn)	0.01	-0.5149	No	Negative	-0.8120
	HLA-DRB101 : 01	KSFYSLPTILTANSD	Consensus (comb.lib./smm/nn)	0.01	-0.0287	No	Negative	-0.4571
	HLA-DRB302 : 02	LVRGNYIGNINNIAR	NetMHCIIpan	0.01	0.8144	No	Positive	0.4925
	HLA-DRB302 : 02	RGNYIGNINNIARND	NetMHCIIpan	0.01	0.8171	Yes	Positive	0.2910
	HLA-DRB101 : 01	TGTKSFYSLPTILTA	Consensus (comb.lib./smm/nn)	0.01	-0.6603	No	Negative	-0.8987
Sera5	HLA-DQA101 : 01/DQB105 : 01	KSYISLFFILCVIFN	Consensus (comb.lib./smm/nn)	0.11	1.0472	No	Negative	-0.086
	HLA-DQA101 : 01/DQB105 : 01	MKSYISLFFILCVIF	Consensus (comb.lib./smm/nn)	0.11	1.1703	No	Positive	0.1021
	HLA-DRB111 : 01	MDIFNNLKRLLIYHS	Consensus (smm/nn/sturniolo)	0.12	-0.1486	No	Negative	1
	HLA-DQA101 : 01/DQB105 : 01	SYISLFFILCVIFNK	Consensus (comb.lib./smm/nn)	0.12	0.7201	No	Positive	0.050
	HLA-DRB111 : 01	EMDIFNNLKRLLIYH	Consensus (smm/nn/sturniolo)	0.18	-0.1137	No	Negative	-0.2916
Sera8	HLA-DRB107 : 01	TLYQLKRVHSNMLIN	Consensus (comb.lib./smm/nn)	0.03	-0.0784	Yes	Negative	-0.0028
	HLA-DRB107 : 01	VTLYQLKRVHSNMLI	Consensus (comb.lib./smm/nn)	0.03	-0.0198	Yes	Positive	0.1241
	HLA-DRB107 : 01	LYQLKRVHSNMLINC	Consensus (comb.lib./smm/nn)	0.06	0.2755	Yes	Negative	1
	HLA-DRB107 : 01	LVTLYQLKRVHSNML	Consensus (comb.lib./smm/nn)	0.07	-0.1590	Yes	Positive	0.1447
	HLA-DRB107 : 01	YQLKRVHSNMLINCF	Consensus (comb.lib./smm/nn)	0.09	-0.3701	No	Negative	1